Validation of Two Rapid Serological Tests for Human Brucellosis Detection


Genc O.
Cetinkol Y.
Buyuktanır Yas O.
Yurdusev N.


As brucellosis have several non-specific clinical phases, the search for reliable diagnosis of the disease is necessary. Therefore, for serological determination of brucellosis, rapid immunofiltration tests designated as ERIFA and NERIFA and ELISAs were developed and evaluated for diagnostic purposes. The sera were collected from Clinical Microbiology Department of Kars State Hospital between 2007 and 2009. These sera were used for determination of sensitivity of ELISAs, ERIFA and NERIFA in comparison to rose bengal test, Brucellacapt and serum agglutination tests. In the study, highest sensitivity was detected by ELISA, ERIFA and NERIFA, respectively. Specificity was highest with ELISA as 95.2% and with both ERIFA and NERIFA as 92.9% and the lowest was with RBT as 90.5%. When two ELISAs were compared based on the IgG sensitivity, there was no difference (p0.05) when IgM was accepted as a criteria for comparison. The current study reports the first evaluation of ERIFA and NERIFA in human beings. It is concluded that the RIFA models can be accepted as an ancillary test for diagnosis of human brucellosis in endemic countries.


How to Cite
O., G., Y., C., O., B. Y., & N., Y. (2019). Validation of Two Rapid Serological Tests for Human Brucellosis Detection. International Journal of Medical and Dental Sciences, 1686–1692.


  1. Corbel MJ. Brucellosis: an overview. Emerg Infect Dis. 1997; 3:213–21. PMid:9204307 PMCid:PMC2627605
  2. Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV. The new global map of human brucellosis. Lancet Infect Dis. 2006; 6:91–9. Available from:
  3. Dean AS, Crump L, Greter H, Schelling E, Zingstagg J. Global burden of human brucellosis: A systematic review of disease frequency. PLOS Neglected Tropical Diseases. 2012; 6(10):1865. DOI: 10.1371/journal.pntd.0001865.
  4. Orduna A, Almaraz A, Prado A, Gutierrez MP, GarciaPascual A, Duenas A, Cuervo M, Abad R, Hernandez B, Lorenzo B, Bratos MA, Torres AR. Evaluation of an ımmunocapture-agglutination test (brucellacapt) for serodiagnosis of human brucellosis. J Clin Microbiol. 2000; 11:4000–5.
  5. Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the brucellosis laboratory. Paris: Institut National de la Recherche Agronomique; 1998.
  6. Yagupsky P. Detection of Brucellae in blood cultures. J Clin Microbiol. 1999; 37:3437–42. PMid:10523530 PMCid:PMC85661
  7. Maichomo MW, McDermott JJ, Arimi SM, Gathura PB. Assessment of the Rose-Bengal plate test for the diagnosis of human brucellosis in health facilities in Narok district, Kenya. East Afr Med J. 1998; 75:219–22. PMid:9745838
  8. Diaz R, Casanova A, Ariza J, Moriyon I. The rose bengal test in human brucellosis: A neglected test for the diagnosis of a neglected disease. Plos Neglected Tropical Diseases. 2011; 5(4):1–7. PMid:21526218 PMCid:PMC3079581
  9. Hewitt WG, Payne DJ. Estimation of IgG and IgM Brucella antibodies in infected and non-infected persons by a radioimmune technique. J Clin Pathol. 1984; 37:692–6.
  10. Parrat D, Nielsen KH, White RG, Payne DJH. Radioimmunoassay of IgM, IgG and IgA Brucella antibodies. Lancet. 1977; 309(8021):1075–8.
  11. Ariza J, Pellicer T, Pallares R, Gudiol F. Specific antibody profile in human Brucellosis. Clin Infect Dis. 1992; 14:131– 40. PMid:1571417
  12. Gomez MC, Nieto JA, Rosa C, Geijo P, Escribano MA, Munoz A, Lopez C. Evaluation of seven tests for the diagnosis of human Brucellosis in an endemic area. Clin Vaccine Immunol. 2008; 15:1031–3. PMid:18448622 PMCid:PMC2446629
  13. Mantur B, Parande A, Amarnath S, Patil G, Walkevar R, Desai A, Parande M, Shinde R, Chandrashekar M, Patil S. ELISA versus conventional methods of diagnosing endemic Brucellosis. Am J Trop Med Hyg. 2010; 83(2):314–8. PMid:20682874 PMCid:PMC2911177
  14. Fadeel MA, Hoffmaster AR, Shi J, Pimentel G, Stoddard RA. Comparison of four commercial IgM and IgG ELISA kits for diagnosing Brucellosis. J Med Microbiol. 2011; 60:1767–73. PMid:21835974
  15. Genç O, Büyüktanır Ö, Yurdusev N. Development of qualitative and quantitative ELISA models for bovine Brucellosis diagnosis. KAU Vet Fak Derg. 2010; 16(2):287–91.
  16. Young EJ. Serologic diagnosis of human brucellosis: analysis of 214 cases by agglutination test and review of the literature. Rev Infect Dis. 1991; 13:359–72. PMid:1866536
  17. Mantecon MA, Gutierrez P, Zarzosa MP, Duenas AI, Solera J, Fernandez-Lago L, Vizcaino N, Almaraz A, Bratos MA, Rodriguez Torres A, Orduna-Domingo A. Utility of an immunocapture-agglutination test and an enzyme-linked immunosorbent assay test against cytosolic proteins from Brucella melitensis B115 in the diagnosis and follow-up of human acute brucellosis. Diagn Microbiol Infect Dis. 2006; 55:27–35. 2005.11.003 PMid:16500069
  18. Moreno MS, Guinea Esquerdo L, Carrero Gonzalez P, Visedo Orden R, Garcia Carbajosa S, Calvo T, Reverte Cejudo D. Diagnosis of brucellosis in an endemic area. Evaluation of routine diagnostic tests. Medicina Clinica (Barcelona). 1992; 98(13):481–5.
  19. Pellicer T, Ariza J, Foz A, Pallares R, Gudiol F. Specific antibodies detected during relapse of human brucellosis. J Infect Dis. 1988; 157(5):918–24. infdis/157.5.918 PMid:3361155
  20. Smits HL, Abdoel TH, Solera J, Clavijo E, Diaz R. Immunochromatographic Brucella-specific immunoglobulin M and G lateral flow assays for rapid serodiagnosis of human brucellosis. Clin Diagn Lab Immunol. 2003; 10: 1141–6.
  21. Genç O, Büyüktanır Ö, Yurdusev N. Development of an individual rapid test based on enzymatic immunofiltration assay for detection of anti–Brucella abortus antibody in bovine sera. J Vet Diagn Invest. 2011; 2:49–56. PMid:21217027
  22. Büyüktanır Ö, Genç O, Çelebi Ö, Yurdusev N. Rapid immunofiltration assay as a field diagnostic tool for ovine brucellosis. J Immun and Immunochem. 2012; 33(1):35–47. PMid:22181819
  23. Gupte S, Kaur T. Diagnosis of human brucellosis. J Trop Dis. 2015; 4(1):1–6.
  24. Bosilkovski M, Spasovska K, Sopova Z, Vidinic I. The role of Brucellacapt test for follow-up patients with brucellosis. Comp Immun Microbiol Infect Dis. 2010; 3:435–42. https:// PMid:19625085
  25. Şahin M, Genç O, Ünver A, Otlu S. Investigation of bovine brucellosis in the Northeastern Turkey. Trop Anim Health Prod. 2008; 40:281–6. PMid:18557191
  26. OIE Terrestrial Manual. Bovine Brucellosis; 2012. p. 630–1.
  27. Büyüktanır Ö, Yıldırım T, Yakıcıer C, Genç O, Yurdusev N. Recombinant PvpA protein-based diagnostic prototype for rapid screening of chicken mycoplasma gallisepticum ınfections. Vet Microbiol. 2008; 129(1-2):139–49. https:// PMid:18248920
  28. Genç O, Büyüktanır Ö, Yurdusev N. Rapid immunofiltration assay based on colloidal gold-protein G conjugate as an alternative screening test for bovine and ovine brucellosis. Tropical Animal Health and Production. 2012; 44(2):213–215. PMid:22102014
  29. Araj GF. Update on laboratory diagnosis of human brucellosis. Int J Antimicrob Agents. 2010; 36:s12–7. PMid:20692128
  30. Geresu MA, Mamo G. Review on diagnostic methods of brucellosis. J Veterinary Sci Techno. 2016; 7:3. DOI: 10.4172/2157-7579.1000323.
  31. Araj GF, Lulu AR, Mustafa MY, Khateeb MI. Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings. J Hyg. 1986; 97:457–69. PMid:3794323
  32. Peeridogaheh H, Golmohammadi MG, Pourfarzi F. Evaluation of ELISA and Brucellacapt tests for diagnosis of human Brucellosis. Iran J Microbiol. 2013; 5(1):14–8. PMid:23467496 PMCid:PMC3577553
  33. del Pozo JSG, Ortuno SL, Navarro E, Solera J. Detection of IgM antibrucella antibody in the absence of IGGS: A challenge for the clinical ınterpretation of brucella serology. PLoS Negl Trop Dis. 2014; 8(12):e3390. PMid:25474572 PMCid:PMC4256177
  34. Arabacı F, Oldacay M. Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. J Microbiol Infect Dis. 2012; 2(2):50–6. ahinjs.02.2012.02.0042
  35. Memish ZA, Almuneef M, Mah MW, Qassem LA, Osoba AO. Comparison of the Brucella standard agglutination test with the ELISA IgG and IgM in patients with Brucella bacteremia. Diagn MicrobiolInfect Dis. 2002; 44:129–32.
  36. Abdoel T, Dias IT, Cardoso R, Smits HL. Simple and rapid field test for brucellosis in livestock. Vet Microbiol. 2008; 130:312–9. PMid:18321664